Interesting event upcoming for parents of incoming international freshmen …

Interesting event upcoming for parents of incoming international freshmen …

Bio senior Mark Pfeiffer and his research poster, “Protein Mutagenesis of ENOX2,” showed well today at the DLRC Intern Poster Session. Pfeiffer is interning at the Purdue Research Park company MorNuCo.
 Pfeiffer said of his work: “My goal was to mutate the amino acid structure of the protein ENOX2. ENOX2 is a known indicator for cancer and is present in a person’s serum. Using silver along with other materials, it is possible to utilize spectroscopic measurements to assess the levels of ENOX2 in a serum sample. The purpose of mutating ENOX2 was to look at the chemical basis for the reaction observed between ENOX2 and silver. It was theorized that a reactive sulfur group on the amino acid cysteine was responsible for the reaction. Protein mutagenesis was achieved using a Quik Change kit to replace a cysteine with an alanine. The protein mutagenesis was found to be successful after both DNA analysis and SDS gels showed the presence of the mutated protein. The mutated protein was then compared to the wild type protein. The mutant showed significant reduction in activity, proving that the amino acid cysteine plays a key role in the chemical reaction.”

Bio senior Mark Pfeiffer and his research poster, “Protein Mutagenesis of ENOX2,” showed well today at the DLRC Intern Poster Session. Pfeiffer is interning at the Purdue Research Park company MorNuCo.


Pfeiffer said of his work: “My goal was to mutate the amino acid structure of the protein ENOX2. ENOX2 is a known indicator for cancer and is present in a person’s serum. Using silver along with other materials, it is possible to utilize spectroscopic measurements to assess the levels of ENOX2 in a serum sample. The purpose of mutating ENOX2 was to look at the chemical basis for the reaction observed between ENOX2 and silver. It was theorized that a reactive sulfur group on the amino acid cysteine was responsible for the reaction. Protein mutagenesis was achieved using a Quik Change kit to replace a cysteine with an alanine. The protein mutagenesis was found to be successful after both DNA analysis and SDS gels showed the presence of the mutated protein. The mutated protein was then compared to the wild type protein. The mutant showed significant reduction in activity, proving that the amino acid cysteine plays a key role in the chemical reaction.”

teded:

The human brain consumes an astounding 3.4 x 10^21 ATP molecules per minute, making it a legitimate energy hog!
From the TED-Ed Lesson What percentage of your brain do you use? - Richard E. Cytowic
Animation by TOGETHER

teded:

The human brain consumes an astounding 3.4 x 10^21 ATP molecules per minute, making it a legitimate energy hog!

From the TED-Ed Lesson What percentage of your brain do you use? - Richard E. Cytowic

Animation by TOGETHER

(via anatomywarehouse)

spaceexp:

Six of the primary mirrors of the James Webb Space Telescope being prepared for acceptance testing

spaceexp:

Six of the primary mirrors of the James Webb Space Telescope being prepared for acceptance testing

(via aluminothermic)

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